The Advanced Light Microscopy Facility, ALM, at Scilifelab gives a two-week PhD-level course on four new imaging and fluorescence spectroscopy techniques:

Depletion-based Super-Resolution Imaging

STED-imaging, 2D and 3D, 30-50 nm resolution
In Living Cells – MoNaLISA, 1-2 Hz, 100 x 100 mm field of view

MINFLUX

Imaging with 3 nm resolution
Tracking single molecules at 10 kHz

Light-Sheet Imaging

Long time-lapse imaging of live model organisms with low phototoxicity
Ultrafast volumetric imaging of cells with lattice light-sheet microscopy

FCS-Methods

Measurements of concentrations, diffusion coefficients, molecular dynamics and interactions, in solution or living cells
Cross-correlation, FRET-, STED-, and line-scan-FCS are covered

Lecturers:

Steven Edwards
Erdinc Sezgin
Ana Agostinho
Francesca Pennacchietti
Hans Blom
Stefan Wennmalm

Credits

3 hp for PhD students

Location: Gamma 3, Scilifelab, Solna

Registration

stewen@kth.se latest September 30th