Contact: stefan.wennmalm@scilifelab.se

Keywords: Super-resolution, Fluorescence Correlation Spectroscopy, MINFLUX, light-sheet imaging

Venue: SciLifeLab Solna

City: Solna

Country: Sweden

Prerequisites:

M.Sc. in any of the Life Sciences or Engineerig Physics, or equivalent

Learning objectives:

-Describe theory and method behind the techniques STED-, MoNaLISA-, and MINFLUX microscopy, light-sheet and lattice light-sheet imaging, the fluctuation method FCS, and the basics of FCCS, FRET-FCS, STED-FCS, and line-scan FCS.
-Explain how these techniques can be applied in their own project and how the central questions in these projects thereby can be answered.
-Compare the capability of the super-resolution techniques with each other and with diffraction-limited techniques.
-Compare the different FCS-techniques.
-Analyze images from the super-resolution and light-sheet techniques. Analyze measurements from the different FCS-techniques.
-Calculate quantities such as field of view, light-sheet thickness, resolution given a certain STED-power, and concentration and molecular brightness in the FCS-techniques.

Target audience: PhD students, postdocs, and researchers at Swedish universities

Tech requirements:

None.

Cost basis: Free to all