New Super-resolution, Light-sheet, and FCS methods at Scilifelab, 3.0 ECTS
Description
Introduction to fluorescence spectroscopy and imaging
Principles of super-resolution microscopy
STED- and RESOLFT-based super-resolution microscopy
The imaging-technique MoNaLISA
The imaging- and tracking-technique MINFLUX
Light-sheet imaging
Lattice light-sheet imaging
Introduction to Fluorescence Correlation Spectroscopy (FCS)
Variants of FCS such as FCCS, STED-FCS, FRET-FCS, and line-scan FCS
Teachers: Ana Agostinho, Erdinc Sezgin, Francesca Pennacchietti, Hans Blom, Stefan Wennmalm, Steven Edwards, Taras Sych
Learning Outcomes
-Describe theory and method behind the techniques STED-, MoNaLISA-, and MINFLUX microscopy, light-sheet and lattice light-sheet imaging, the fluctuation method FCS, and the basics of FCCS, FRET-FCS, STED-FCS, and line-scan FCS.
-Explain how these techniques can be applied in their own project and how the central questions in these projects thereby can be answered.
-Compare the capability of the super-resolution techniques with each other and with diffraction-limited techniques.
-Compare the different FCS-techniques.
-Analyze images from the super-resolution and light-sheet techniques. Analyze measurements from the different FCS-techniques.
-Calculate quantities such as field of view, light-sheet thickness, resolution given a certain STED-power, and concentration and molecular brightness in the FCS-techniques.
Prerequisites & Technical Requirements
Prerequisites
M.Sc. in any of the Life Sciences or Engineerig Physics, or equivalent
Technical requirements
None.
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