Description

Participants will practice TEM sample preparation and TEM imaging with a sample from their own project. Lab space limits the number of participants to 8, chosen by motivation and project aim. Write a short project description, motivation for participating and what sample you would like to bring for the course. It takes time to do fixation and plastic embedding. Therefore, one test-sample should be brought to be prepared before the course, and a new test-sample is brought for the first day.

In addition, a small presentation about an EM-project idea of your own should be prepared and presented on the last day.

Participants should attend all theoretical and practical parts of the course to receive a certificate of 1,5 hp.

This is a practical course on biological sample preparation for TEM and includes:
-Ultra-microtome sectioning
-Chemical fixation
-Plastic embedding
-TEM imaging
-High-pressure freezing & freeze substitution
-Immunolabeling techniques (Immunogold, CLEM)
-Volume EM (FIB-SEM & Tomography)
-Segmentation and sampling - Online lecture by Ilya Belevich, University of Helsinki

Event Details

Dates
8 - 12 December 2025
Application deadline
November 23, 2025 23:59
Contact

Sara Henriksson
sara.henriksson@umu.se

Agnieszka Ziolkowska
agnieszka.ziolkowska@umu.se

Venue
UCEM facility (EM-building) and Chemical Biological Centre (KBC) building, Umeå University, Linnaeus väg 6
City
Umeå
Country
Sweden
Language
English
Cost
Free to all
Timezone
Stockholm

Learning Outcomes

A practical course on biological sample preparation for TEM

Prerequisites & Technical Requirements

Prerequisites

Basic EM course at UCEM or previous equal experiences with EM methods


Technical requirements

Samples from their own project, one test-sample should be brought to be prepared before cours, and a new one should be prepared and presented on the last day.

Small presentation about an EM-project idea of your own

Topics & Tags

Keywords
TEMsample preparationbiological samplepractical courseultra-microtome sectioningChemical fixationPlastic embeddingTEM imagingSegmentation & samplingVolume EMImmunolabeling techniquesHigh-pressure freezingfreeze substitution
Target audience
PhD studentspostdocsresearchers

Affiliations & Networks

SciLifeLab affiliation
Yes
Associated nodes
SciLifeLab

Activity log