Proteomics by Mass Spectrometry: When and How
Date: 3 - 14 November 2025
The course "Mass Spectrometry based Proteomics: When and How" is a 2-week course designed for PhD students or those at an equivalent level who wish to gain comprehensive knowledge about mass spectrometry based proteomics. This course will offer a wide-ranging introduction to the field of mass spectrometry based proteomics, benefiting newcomers while also delving into the advantages and disadvantages of various methods to provide more experienced users with substantial insights.
Apply here!
Course content
Practical Laboratory Exercise: Practical laboratory exercise utilizes mass spectrometry-based proteomics to teach sample preparation, demonstrate sample acquisition and analysis, and provide a foundation for basic data interpretation.
Skills Acquisition and Project Presentation: The skills acquired during the course will be applied to write and orally present a brief proteomics research project.
Focus of the Course: This course emphasizes proteomics technologies and applications. For proteomics data analysis, we recommend our KI doctoral course "Omics Data Analysis: From Quantitative Data to Biological Information”
Lectures and workshops on:
- Overview of proteomics
- The proteomics workflow
- Basic of separation sciences: Protein and peptides
- Sample preparation for proteomics
- Introduction to Mass spectrometry
- Quantitative proteomics methods
- Global and targeted proteomic
- Experimental design
- Plasma proteomics
- Protein Identification statistics
- Proteogenomics
- Biological validation
- Clinical applications
- Single cell proteomics
- Data Analysis & Bioinformatics
Selection criteria
Selection will be based on:
1) the relevance of the course syllabus for the applicant’s individual study plan/research (according to written motivation).
2) start date of doctoral studies (priority given to earlier start date).
Contact: henrik.johansson@ki.se
Keywords: proteomics, omics, mass spectrometry
Venue: Department of Oncology-Pathology, Karolinska Institute-SciLifeLab
City: Stockholm
Country: Sweden
Prerequisites:
While no specific prerequisite courses are necessary for this program, a basic understanding at the university level of molecular biology, chemistry, biochemistry, and omics technologies is recommended to fully benefit from the course.
Learning objectives:
After completing the course, the student will be able to:
-Define and apply common proteomics terminology
-Recognize the principles of the most common proteomics techniques
-Describe the mass spectrometry based proteomics workflow: Experimental design, sample preparation and selection of techniques
-Understand the basic principles of MS based proteomics from sample preparation to generation of the protein data matrix to such an extent that they will be able to utilize proteomics core facilities or collaborate with proteomics researchers.
-Evaluate the quality of protein and peptide identifications.
-Give examples on how modern proteomics is applied to clinical research and to evaluate when proteomics research could be beneficial to incorporate in research projects.
-Design your own research project by mass spectrometry based proteomics.
Target audience: PhD Students
Event types:
- Workshops and courses
Activity log
