Single-Cell RNA-Seq Data Analysis
Description
PLEASE NOTE! This is a 5-day course. The first part (lectures and practicals) takes place 13-16 March. The second part (bring your own data session) takes place 27 March.
This workshop will introduce the best practice bioinformatics methods for analyzing single cell RNA-seq data via a series of lectures and computer practicals (using R or Python according to each student’s preference). The total course duration is 5 days, which includes 4 days of lectures and exercises followed by a "Bring Your Own Data" (BYOD) day where you get to practice your skills on a dataset of your choosing. Please note that the BYOD section will take place two weeks after the lectures.
Topics covered will include:
- Overview of the current scRNAseq technologies
- Basic overview of pipelines for processing raw reads into expression values
- Quality control and normalization
- Dimensionality reduction techniques
- Data integration and batch correction
- Differential gene expression
- Clustering techniques
- Cell type prediction
- Trajectory inference analysis
- Comparison of Seurat, Bioconductor and Scanpy toolkits
This online training event has no fee for academic participants. However, if you accept a position at the workshop and do not participate (no-show) you will be invoiced 3000 SEK. For non-academic participants, the course fee (and no-show fee) is 12000 SEK. Please note that NBIS cannot invoice individuals.
Learning Outcomes
Upon completion of this course, you will be able to run analysis on your own data, select appropriate analysis methods and interpret the results.
Prerequisites & Technical Requirements
Prerequisites
The following is a list of skills required for being able to follow the course and complete the exercises:
- You are familiar with basic UNIX command line (bash)
- You are familiar with R and/or Python
- You understand the basics of NGS technologies
- You are familiar with analyses of bulk RNA-sequencing data
The course accommodates a maximum of 25 participants. If we receive more applications, participants will be selected based on selection criteria including (but not limited to) correct entry requirements, motivation to attend the course, as well as gender and geographical balance. Priority is given to PhD candidates followed by other researchers. International applicants are accepted based on availability of seats.
Technical requirements
You will need to have full access to your computer (admin permissions). Instructions on installation will be sent by email to accepted participants.
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