Biodiversity Genomics Europe + SciLifeLab Digital Hi-C Course
Description
With the collaboration of five research institutes, two industry partners, and many Universities, three streams of the HiC technique were filmed in 2024 onsite at BMC Uppsala. These have been digitized and are now available as a digital course, openly available to all.
Aim
The aim of this course is to deliver an open, sustainable training in laboratory preparation of Hi-C libraries for reference genome sequencing of non-model organisms for BGE partner sequencing centers.
Course contents
- In the Introductory stream, researchers with no previous experience of the HiC technique are guided in performing the Arima HiC protocol for the first time.
- In the Challenging Samples stream, Hi-C experts contribute specialized knowledge and experience while performing the Arima HiC protocol on challenging samples.
- In the AssemblyLink stream, researchers with previous experience in the Hi-C technique perform and troubleshoot a variety of samples with the AssemblyLink protocol.
Upcoming Training Instances
No upcoming training instances.
Learning Outcomes
By the end of the course, participants will be able to:
- independently prepare HiC libraries of low-complexity samples.
- prepare HiC libraries at their home labs.
- understand the most crucial steps of the protocol
- troubleshoot crucial steps of the protocol in their home labs when applied to high-complexity samples
Structure & Duration
Introductory stream - 2h40m video content
Challenging Sample stream - 3h20m video content
AssemblyLink stream - 2h30m video content
Discussion content - 3h15m video content
Data from sample preparation is also available.
Prerequisites & Technical Requirements
Prior Knowledge
Participants must have:
an understanding of fundamental genomics and epigenomics concepts
the ability to demonstrate basic laboratory technique, including safe laboratory practices
Participants should have:
an understanding of their local permit and safety requirements for processing and handling samples and reagents for HiC.
Technical Requirements
None
Audience & Keywords
Target Audience
Keywords
Activity log
