The purpose of the course is to prepare and train cryoEM facility users in sample preparation methods, introduce users to the image data acquisition workflow, expand knowledge about cryo-EM methods among researchers and show that everyone can learn how to use cryo-EM for structural biology.
The course is open for facility users or potential facility users, such as PhD students, postdocs, and researchers within the life sciences who are curious and will profit from cryo-EM skills. We welcome local and international applications. To attend, the course participants should be familiar with electron microscopy and structural biology.
This course is supported by SciLifeLab, CryoNET and Umeå University and organized by the Umeå Centre for Electron Microscopy (UCEM).

This course will cover practical aspects of cryo-EM sample preparation and data acquisition.

The following topics will be covered:
Overview of cryo-EM methods

Microscope design and electron optics

Sample preparation

Data collection for single particle analysis (SPA)
Introduction to SPA image processing

The course consists of lectures, cryo lab work, demonstrations and practical exercises for all participants, hands-on experiences of microscopy operation and discussions. A detailed schedule will be provided to accepted participants.